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EFSA FEEDAP — L-tryptophan (C. glutamicum KCCM 80346, GM) for all animal species: safe; ruminant pulmonary-injury risk from unprotected dose; sensitiser (EFSA Journal 2025;23(12):e9795)

EFSA_FEEDAP_ltryptophan_safety

efsa 450 tok en 2026-08-06

EFSA FEEDAP — L-tryptophan (C. glutamicum KCCM 80346, GM) for all animal species

Source: EFSA FEEDAP scientific opinion, EFSA Journal 2025;23(12):e9795 (DOI 10.2903/j.efsa.2025.9795), retrieved 2026-08-06 via Europe PMC OA full-text XML. Paraphrase of an EFSA opinion that the source XML tags CC-BY-ND; per project COPYRIGHT_POLICY only facts/numbers/proper-nouns are quoted verbatim, sentences rewritten.

Editorial context (read this before using the data point)

This is the EFSA FEEDAP jurisdiction on the amino-acid feed additive C. glutamicum KCCM 80346 — one of four amino-acid safety opinions in this batch. Read with the efsa nutrient endpoints and AAFCO/FEDIAF amino-acid provisions.

Key findings (paraphrase; numbers and proper nouns verbatim from source)

  • L-tryptophan is produced using the genetically modified strain *C. glutamicum* KCCM 80346; characterised up to 94.9%, so conclusions apply only to that manufacturing process.
  • Its use in animal nutrition is considered safe for all animal species and safe for the consumer and for the environment.
  • There may be a risk in ruminants (pulmonary injury) when unprotected tryptophan is used, linked to increased production of the pneumotoxic metabolite 3-methylindole (skatole).
  • For user safety it is not irritant to skin or eyes but should be considered a skin and respiratory sensitiser; inhalation and dermal exposure are a risk.
  • It is an effective source of the essential amino acid L-tryptophan for all animal species; to match non-ruminant efficacy in ruminants it should be protected from ruminal degradation.

Pet-food relevance

L-tryptophan is an essential amino acid in cats and dogs. The broad 'safe for all animal species' conclusion is the headline; the ruminant pulmonary-injury caveat is the key species-specific limitation. The ~5% unidentified fraction (fermentation residues) is the stated residual uncertainty.

Scope note / honest gap

Paraphrase of an EFSA opinion; source XML licence tag was CC-BY-ND, so this topic quotes only data points verbatim and rewrites prose. Full genotoxicity and sub-chronic study details are not reproduced.

Sources

EFSA FEEDAP — L-tryptophan (C. glutamicum KCCM 80346, GM) for all animal species: safe; ruminant pulmonary-injury risk from unprotected dose; sensitiser (EFSA Journal 2025;23(12):e9795)
Source document: EFSA FEEDAP — L-tryptophan (C. glutamicum KCCM 80346, GM) for all animal species: safe; ruminant pulmonary-injury risk from unprotected dose; sensitiser (EFSA Journal 2025;23(12):e9795)
EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)EFSA Journal 2025;23(12):e9795retrieved 2026-08-06

Verification file: pdf-raw/efsa_l/PMC12706517.xml